Multiplex Quantitative NanoImaging of Synaptic Proteins

DaeWon Moon
DGIST, Korea
16:00~16:45,November 20th, 2015


Recent developments on imaging mass spectrometry such as MALDI (matrix-assisted laser desorption and ionization) and SIMS (Secondary Ion Mass Spectrometry) have provided ultrahigh content molecular information on cells and tissues. However, MALDI imaging with ~ 10-micrometer spatial resolution cannot provide subcellular imaging and SIMS in spite of its excellent spatial resolution of ~ 1 micrometer or even better cannot provide protein imaging due to its destructive feature. Utilizing high secondary ion yields of metal oxide nanoparticles, proteins can be SIMS imaged with metal oxide nanoparticle tagged proteins. Various metal oxide nanoparticles have been tried and shown to be useful for protein imaging such as SiO2, Fe3O4, TiO2, PbS, and In2O3. Synaptic proteins tagged with metal oxide nanoparticles such as Ach, AMPA, PSD95, Cb1 were imaged simultaneously with SIMS for hippocampus tissues. Lipid and neurotransmitter images can be obtained simultaneously with protein images for deeper understanding of neurobiology, which is a unique capability among other bioimaging techniques. The protein images from TOF-SIMS were compared with confocal fluorescence microscopy in a good agreement with complementary features

The areal density of metal oxide nanoparticles can be determined quantitatively with TOF-MEIS (time-of-flight medium energy ion scattering), which can be used for determination of the absolute areal density of proteins. Additionally, single metal oxide nanoparticles of 10 nm diameter can be imaged with HIM (He Ion Microscopy)

Combining metal oxide tagged proteins, SIMS, TOF-MEIS, HIM all together, we developed a power and unique nanobio imaging platform methodology for multiplex and quantitative protein nanoimaging with single protein resolution, which should be utilized for a wide range of biomedical problems.



Research Interests:

  • Quantitative and Imaging Analysis of Nano & Bio Interfaces
  • Medium Energy Ion Scattering Spectroscopy for nm thin films, nanoparticles, and
    electric double layer
  • Secondary Ion Mass Spectrometry for biosurface analysis
  • Coherent Anti-Stokes Raman Scattering for Bio-imaging
  • Ambient mass imaging for live cells analysis



Honors & Prizes:

  • 2010-present Co-editor, Biointerphase
  • 2006-present Member, Korean Academy of Science and Technology
  • 2003-present Editorial Board, Critical Reviews in Solid State and Materials Science
  • 2013 Chair, International SIMS conference, Jeju, Korea
  • 2011 Best Paper Award, Korean Federation of Science and Technology Societies
  • 2011 Best Research Award, Ministry of Knowledge Economy, Korea
  • 2010 Academic Research Award, Korea Vacuum Society
  • 2008 KRISS fellow
  • 2005 National Science and Technology Medal (Do-Yak-Jang), Korea Government
  • 2004 Award of Best Ten Scientists in 2004 by MOST and Dong-A Press, Korea
  • 2001 Award of Ministry of Science and Technology ‘Scientist of This Month’


Professional Activities:


  • 2012-present DGIST Fellow, Professor of Major in New Biology, DGIST
  •  2003-2012 Professor, Department of NanoBio Surface Science, UST(University of Science and Technology)
  • 2006-2010 Head, Center for Nano-Bio Technology, KRISS
  • 1998-2006 Group Leader, NanoSurface Group, KRISS
  • 2001-2002 Visiting Professor, Dept. of Materials Science, University of Illinois, Urbana Champaign, USA
  • 1986-2012 Principal Researcher, KRISS.
  • 1984-1986 Research Associate, Princeton University, USA